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Gel Permeation Chromatography

Size Exclusion Chromatography (SEC)


  • Method:
    Determination of average molecular weight and weight distribution through fractionation on polymer gel columns through comparison with calibration standards



  • Determination of:
    Number average molecular weight Mn
    Weight average molecular weight Mw
    Molecular weight distribution Polydispersity PDI=Mw/Mn

  • Equipment:
    5 units working on different solvent
    1 high-temperature SEC (up to 150 °C)

  • Literature:
    Hunt, G. J. ; Size Exclusion Chromatography; Chapman & Hall, New York, 1979
    Yau, W.W.; J. J. Kirkland; D. D. Bly; Modern Size Exclusion Chromatography, J. Wiley & Sons, New York, 1979
    Dublin, P.L.; Aqueous Size-Exclusion Chromatograpy; Journal of Chromatography Lib. 40, Elsevier, 1988


    • Contact:   Sandra Seywald Ute Heinz Christine Rosenauer
    Phone: 06131-379 - 227       06131-379 - 227       06131-379 - 225

    letzte Änderung: 10.2.2010
    contact: Rainer Lösch (Tel: 06131-379-225)